Year 2017, Volume 4 , Issue 2, Pages 103 - 113 2017-04-23

Investigation of the Effects of 2,4-Dimethylpyrrole on Fusarium culmorum Causal Agent of Head Blight Disease
2,4-Dimetilpirol’ün Başak Yanıklığı Hastalığı Etmeni Fusarium culmorum’un Üzerine Etkilerinin İncelenmesi

Özlem SEFER [1] , Emre YÖRÜK [2] , Elif Sedef DEVELİ [3] , Ayşe Server SEZER [4] , Zeynep KONUKCU [5]


Fusarium culmorum, a phytopathogen, cause several diseases including root rot and head blight on cereals in worldwide and our country. In this study, effects of 2,4-Dimethylpyrrole, as a novel and potential agent on pathogen which can be used in fight with F. culmorum were investigated. For this purpose, alteration in linear growth rate (LGR) in F. culmorum 20F isolate, subjected to different concentrations of 2,4-Dimethylpyrrole (0, 0.5, 1, 2, 4 mg mL-1) was investigated and the significant decrase between control and experiment sets was determined. Besides, expression of Mgv1 gene, responsible for sexual stage and cell wall integrity, was investigated via quantitative real time polymerase chain reaction (q-PCR) and reverse transcription PCR (RT-PCR). In comparison to control groups, 5.21±0.05x102 fold increases in experiment set for Mgv1 gene was detected. According to findings obtained, it was shown that 2,4-Dimethylpyrrole could be a potential antifungal agent for F. culmorum. In further works, it is suggested that this agent could be used as a novel approach in disease control including inhibition of fungal biomass and toxin production in field.

Bitki patojeni Fusarium culmorum dünyada ve ülkemizde tahıllar üzerinde kök çürüklüğü ve başak yanıklığı başta olmak üzere çeşitli hastalıklara neden olmaktadır. Bu çalışmada F. culmorum ile mücadele kullanılabilecek yeni ve potansiyel bir ajan olarak 2,4-Dimetilpirol’ün patojen üzerindeki etkileri incelenmiştir. Bu amaçla farklı konsantrasyonlarda 2,4-Dimetilpirol (0, 0.5, 1, 2 ve 4 mg mL-1) uygulanmış F. culmorum 20F izolatında artan konsantrasyonlarda doğrusal büyüme oranındaki (DBO)  değişim incelenmiş ve kontrol ve deney grupları arasında anlamlı bir düşüş olduğu görülmüştür.  Ayrıca seksüel üreme ve hücre çeperi bütünlüğünden sorumlu Mgv1 geninin anlatımı gerçek zamanlı kantitatif polimeraz zincir reaksiyonu (k-PZR) ve ters transkripsiyon PZR (RT- PZR) ile incelenmiştir. Deney gruplarında kontrol gruplarına göre Mgv1 geni için 5.21±0.05x102 kat artma saptanmıştır. Elde edilen bulgulara göre 2,4-Dimetilpirol’ün, F. culmorum türünde potansiyel bir antifungal etkili ajan olabileceği gösterilmiştir. İleriki çalışmalarda bu ajanın tarladaki fungal biyokütlenin ve toksin üretiminin azaltılmasını sağlayarak hastalık ile mücadelede yeni bir yaklaşım olarak kullanılabileceği düşünülmektedir.

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Subjects Biology
Published Date July
Journal Section Articles
Authors

Author: Özlem SEFER
Institution: İSTANBUL YENİ YÜZYIL ÜNİVERSİTESİ
Country: Turkey


Author: Emre YÖRÜK
Institution: İSTANBUL YENİ YÜZYIL ÜNİVERSİTESİ
Country: Turkey


Author: Elif Sedef DEVELİ
Institution: İSTANBUL YENİ YÜZYIL ÜNİVERSİTESİ
Country: Turkey


Author: Ayşe Server SEZER
Institution: İSTANBUL YENİ YÜZYIL ÜNİVERSİTESİ
Country: Turkey


Author: Zeynep KONUKCU
Institution: İSTANBUL YENİ YÜZYIL ÜNİVERSİTESİ
Country: Turkey


Dates

Publication Date : April 23, 2017

Bibtex @research article { ijsm307943, journal = {International Journal of Secondary Metabolite}, issn = {}, eissn = {2148-6905}, address = {Prof. Dr. İzzet KARA}, publisher = {İzzet KARA}, year = {2017}, volume = {4}, pages = {103 - 113}, doi = {10.21448/ijsm.307943}, title = {Investigation of the Effects of 2,4-Dimethylpyrrole on Fusarium culmorum Causal Agent of Head Blight Disease}, key = {cite}, author = {SEFER, Özlem and YÖRÜK, Emre and DEVELİ, Elif Sedef and SEZER, Ayşe Server and KONUKCU, Zeynep} }
APA SEFER, Ö , YÖRÜK, E , DEVELİ, E , SEZER, A , KONUKCU, Z . (2017). Investigation of the Effects of 2,4-Dimethylpyrrole on Fusarium culmorum Causal Agent of Head Blight Disease. International Journal of Secondary Metabolite , 4 (2) , 103-113 . DOI: 10.21448/ijsm.307943
MLA SEFER, Ö , YÖRÜK, E , DEVELİ, E , SEZER, A , KONUKCU, Z . "Investigation of the Effects of 2,4-Dimethylpyrrole on Fusarium culmorum Causal Agent of Head Blight Disease". International Journal of Secondary Metabolite 4 (2017 ): 103-113 <http://ijsm.ijate.net/en/issue/27381/307943>
Chicago SEFER, Ö , YÖRÜK, E , DEVELİ, E , SEZER, A , KONUKCU, Z . "Investigation of the Effects of 2,4-Dimethylpyrrole on Fusarium culmorum Causal Agent of Head Blight Disease". International Journal of Secondary Metabolite 4 (2017 ): 103-113
RIS TY - JOUR T1 - Investigation of the Effects of 2,4-Dimethylpyrrole on Fusarium culmorum Causal Agent of Head Blight Disease AU - Özlem SEFER , Emre YÖRÜK , Elif Sedef DEVELİ , Ayşe Server SEZER , Zeynep KONUKCU Y1 - 2017 PY - 2017 N1 - doi: 10.21448/ijsm.307943 DO - 10.21448/ijsm.307943 T2 - International Journal of Secondary Metabolite JF - Journal JO - JOR SP - 103 EP - 113 VL - 4 IS - 2 SN - -2148-6905 M3 - doi: 10.21448/ijsm.307943 UR - https://doi.org/10.21448/ijsm.307943 Y2 - 2017 ER -
EndNote %0 International Journal of Secondary Metabolite Investigation of the Effects of 2,4-Dimethylpyrrole on Fusarium culmorum Causal Agent of Head Blight Disease %A Özlem SEFER , Emre YÖRÜK , Elif Sedef DEVELİ , Ayşe Server SEZER , Zeynep KONUKCU %T Investigation of the Effects of 2,4-Dimethylpyrrole on Fusarium culmorum Causal Agent of Head Blight Disease %D 2017 %J International Journal of Secondary Metabolite %P -2148-6905 %V 4 %N 2 %R doi: 10.21448/ijsm.307943 %U 10.21448/ijsm.307943
ISNAD SEFER, Özlem , YÖRÜK, Emre , DEVELİ, Elif Sedef , SEZER, Ayşe Server , KONUKCU, Zeynep . "Investigation of the Effects of 2,4-Dimethylpyrrole on Fusarium culmorum Causal Agent of Head Blight Disease". International Journal of Secondary Metabolite 4 / 2 (April 2017): 103-113 . https://doi.org/10.21448/ijsm.307943
AMA SEFER Ö , YÖRÜK E , DEVELİ E , SEZER A , KONUKCU Z . Investigation of the Effects of 2,4-Dimethylpyrrole on Fusarium culmorum Causal Agent of Head Blight Disease. IJSM. 2017; 4(2): 103-113.
Vancouver SEFER Ö , YÖRÜK E , DEVELİ E , SEZER A , KONUKCU Z . Investigation of the Effects of 2,4-Dimethylpyrrole on Fusarium culmorum Causal Agent of Head Blight Disease. International Journal of Secondary Metabolite. 2017; 4(2): 113-103.