Year 2019, Volume 6 , Issue 1, Pages 28 - 37 2019-03-16

Determination of anticancer effects of Urospermum picroides against human cancer cell lines

Mehlika Alper [1] , Hatice Güneş [2]


Plants continue to be a good source for developing effective anticancer agents. In this study, in vitro various biological effects of crude ethanolic extract from flowering parts of Urospermum picroides collected from the Muğla province of Turkey were investigated for the first time. Daudi, A549 and HeLa cancer cell lines and BEAS-2B normal cell line were used to identify the cytotoxic effect of the extract using MTT assay. The effect of the extract on cell cycle progression was detected by flow cytometric analysis. The level of VEGF, IL-1α, IL-6 and TNF-α secretion in the cells treated with the extract were measured using ELISA The extract caused a higher cytotoxic effect on Daudi cells with an IC50 value of 85.64 µg/mL than the other cells tested. The IC50 values in HeLa and A549 cells were determined to be 135.35 and 234.8 µg/ mL, respectively. The selective cytotoxicity was considered between Daudi and BEAS-2B (109.80 µg/mL) cell lines. In addition, the effect of the extract on cell cycle progression changes according to cell line used. Moreover, the extract decreased the level of secreted VEGF in treated A549 cells by 31%. In addition, the extract resulted in a significant decrease in the secretion of IL-1α, IL-6 and TNF-α cytokines in A549 and Daudi cells compared to the untreated cells. These findings suggest that the flowering parts of U. picroides may be a potential source for anticancer agents.
Urospermum picroides, cytotoxicity, cell cycle analysis, cancer cell lines
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Primary Language en
Subjects Biology
Published Date March
Journal Section Articles
Authors

Orcid: 0000-0001-6193-346X
Author: Mehlika Alper
Institution: MUGLA SITKI KOCMAN UNIVERSITY, FACULTY OF SCIENCE, DEPARTMENT OF MOLECULAR BİOLOGY and GENETICS
Country: Turkey


Orcid: 0000-0001-5191-365X
Author: Hatice Güneş (Primary Author)
Institution: MUGLA SITKI KOCMAN UNIVERSITY, FACULTY OF SCIENCE, DEPARTMENT OF BİOLOGY
Country: Turkey


Dates

Publication Date : March 16, 2019

Bibtex @research article { ijsm482404, journal = {International Journal of Secondary Metabolite}, issn = {}, eissn = {2148-6905}, address = {Prof. Dr. İzzet KARA}, publisher = {İzzet KARA}, year = {2019}, volume = {6}, pages = {28 - 37}, doi = {10.21448/ijsm.482404}, title = {Determination of anticancer effects of Urospermum picroides against human cancer cell lines}, key = {cite}, author = {Alper, Mehlika and Güneş, Hatice} }
APA Alper, M , Güneş, H . (2019). Determination of anticancer effects of Urospermum picroides against human cancer cell lines. International Journal of Secondary Metabolite , 6 (1) , 28-37 . DOI: 10.21448/ijsm.482404
MLA Alper, M , Güneş, H . "Determination of anticancer effects of Urospermum picroides against human cancer cell lines". International Journal of Secondary Metabolite 6 (2019 ): 28-37 <http://ijsm.ijate.net/en/issue/41604/482404>
Chicago Alper, M , Güneş, H . "Determination of anticancer effects of Urospermum picroides against human cancer cell lines". International Journal of Secondary Metabolite 6 (2019 ): 28-37
RIS TY - JOUR T1 - Determination of anticancer effects of Urospermum picroides against human cancer cell lines AU - Mehlika Alper , Hatice Güneş Y1 - 2019 PY - 2019 N1 - doi: 10.21448/ijsm.482404 DO - 10.21448/ijsm.482404 T2 - International Journal of Secondary Metabolite JF - Journal JO - JOR SP - 28 EP - 37 VL - 6 IS - 1 SN - -2148-6905 M3 - doi: 10.21448/ijsm.482404 UR - https://doi.org/10.21448/ijsm.482404 Y2 - 2019 ER -
EndNote %0 International Journal of Secondary Metabolite Determination of anticancer effects of Urospermum picroides against human cancer cell lines %A Mehlika Alper , Hatice Güneş %T Determination of anticancer effects of Urospermum picroides against human cancer cell lines %D 2019 %J International Journal of Secondary Metabolite %P -2148-6905 %V 6 %N 1 %R doi: 10.21448/ijsm.482404 %U 10.21448/ijsm.482404
ISNAD Alper, Mehlika , Güneş, Hatice . "Determination of anticancer effects of Urospermum picroides against human cancer cell lines". International Journal of Secondary Metabolite 6 / 1 (March 2019): 28-37 . https://doi.org/10.21448/ijsm.482404
AMA Alper M , Güneş H . Determination of anticancer effects of Urospermum picroides against human cancer cell lines. IJSM. 2019; 6(1): 28-37.
Vancouver Alper M , Güneş H . Determination of anticancer effects of Urospermum picroides against human cancer cell lines. International Journal of Secondary Metabolite. 2019; 6(1): 37-28.