Year 2019, Volume 6 , Issue 2, Pages 154 - 161 2019-07-15

Antioxidant Activities, Phenolic Contents and Electronic Nose Analysis of Black Garlic

Ozan Emre Eyupoglu [1]


Black garlic is a processed garlic product with a moisture-controlled high temperature heat treatment for a long time. In order to determine the secondary metabolites of black garlics treated in the study, firstly, in vitro antioxidant activities of black garlics purchased from Edovital company, Kastamonu, Turkey were determined, followed by qualitative and quantitative measurement of the phenolic compound content by HPLC and finally the electronic nose analysis of the content of nebulizer vapors in wood vinegar extract of black garlics were done successfully. Chlorogenic acid, vanillic acid, benzoic acid, gallic acid contents in detected 13 phenolic acids were quitely high. All quantitative results were expressed as mg gallic acid equivalent (GAE) per g dry matter of black garlic sample. ABTS and DPPH antioxidant activities were very low according to BHT standart and 2-Methylene-4-pentenal (18%) and Furfural (25%) were detected in high amount with electronic nose in nebulvapor contents of black garlic wood vinegar extract.
Black garlic, Antioxidant activity, Phenolic contents, Electronic nose, HPLC analysis
  • [1]. Lu, X., Li, N., Qiao, X., Qiu, Z., and Liu, P. (2017). Composition analysis and antioxidant properties of black garlic extract. J. Food Drug Anal. 25, 340–349, doi: 10.1016/j.jfda.2016.05.011.
  • [2]. Sun, Y. E., and Wang, W. (2018). Changes in nutritional and bio-functional compounds and antioxidant capacity during black garlic processing. J. Food Sci. Technol. 55, 479–488, doi: 10.1007/s13197-017-2956-2
  • [3]. Dong, M., Yang, G., Liu, H., Liu, X., Lin, S., Sun, D., et al. (2014). Aged blackgarlic extract inhibits ht29 colon cancer cell growth via the pi3k/akt signaling pathway. Biomed. Rep. 2, 250–254, doi: 10.3892/br.2014.226
  • [4]. Czompa, A., Szoke, K., Prokisch, J., Gyongyosi, A., Bak, I., Balla, G., et al. (2018). Aged (black) versus raw garlic against ischemia/reperfusion-induced cardiac complications. Int. J. Mol. Sci. 19 (4), 1017, doi: 10.3390/ijms19041017
  • [5]. Kimura, S., Tung, Y. C., Pan, M. H., Su, N. S., Lai, Y. J., & Cheng, K. C. (2017). Black garlic: A critical review of its production, bioactivity, and application. J. Food Drug Anal., 25, 62–70.
  • [6]. Queiroz, Y.S, Ishimoto, E.Y, Bastos, D.H.M, Sampaio, G.R, Torres, E.A.F.S. (2009). Garlic (Allium sativum L.) and ready-to-eat garlic products: in vitro antioxidant activity. Food Chem., 115, 371-374.
  • [7]. Choi, I.S, Cha, H.S, Lee, Y.S. (2014). Physicochemical and antioxidant properties of black garlic. Molecules, 19, 16811-16823.
  • [8]. Seo, Y.J, Gweon, O.C., Im, J., Lee, Y.M., Kang, M.J., Kim, J.I. (2009). Effect of garlic and aged black garlic on hyperglycemia and dyslipidemia in animal model of type 2 diabetes mellitus. J Food Sci Nutr, 14, 1-7.
  • [9]. Yuan, H., Sun, L., Chen, M., Wang, J. (2016). The comparison of the contents of sugar, Amadori, and Heyns compounds in fresh and black garlic. J Food Sci, 81(7), C1662-C1668.
  • [10]. Bae, S.E., Cho, S.Y., Won, Y.D., Lee, S.H., & Park, H.J. (2012). A comparative study of the different analytical methods for analysis of S-allyl cysteine in black garlic by HPLC. LWT-Food Sci. Technol. (Campinas), 46, 532–535.
  • [11]. Molina-Calle, M., Priego-Capote, F., & Luque de Castro, M. D. (2016). HS-GC/MS volatile profile of different varieties of garlic and their behavior under heating. Anal Bioanal Chem, 408 (14), 3843–3852, doi:10.1007/s00216-016-9477-0
  • [12]. Amagase, H., et al. (2001). Intake of garlic and its bioactive components. The Journal of Nutrition, 131, 955S–962S.
  • [13]. Liu J., Zhang G., Cong X., Wen C. (2018). Black Garlic Improves Heart Function in Patients With Coronary Heart Disease by Improving Circulating Antioxidant Levels. Front. Physiol., 9 (1435), 1-11, doi: 10.3389/fphys.2018.01435
  • [14]. Jeong, Y., Ryu, J., Shin, J.-H., Kang, M., Kang, J., Han, J., & Kang, D. (2016). Comparison of Anti-Oxidant and Anti-Inflammatory Effects between Fresh and Aged Black Garlic Extracts. Molecules, 21(4), 430, doi:10.3390/molecules21040430
  • [15]. Chen Y.-C., Kao T.-H., Tseng C.-Y., Chang W.-T., Hsu C.-L. (2014). Methanolic extract of black garlic ameliorates diet-induced obesity via regulating adipogenesis, adipokine biosynthesis, and lipolysis. J Funct Foods, 9, 98-108, doi:10.1016/j.jff.2014.02.019
  • [16]. Zhang, R.F., Zhang, F.X., Zhang, M.W., Wei, Z.C., Yang, C.Y., Zhang, Y. (2011). Phenolic composition and antioxidant activity in seed coats of 60 Chinese black soybean (Glycine max L. Merr.) varieties. J. Agric. Food Chem., 59, 5935–5944.
  • [17]. Benzie, I.F.F., and Strain, J.J. (1996). The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: The FRAP assay. Anal. Biochem., 239, 70-76.
  • [18]. Ozyurek, M., Guclu, K., Apak, R. (2011). The main and modified CUPRAC methods of antioxidant measurement. Trends Anal. Chem., 30 (4), 652-664, doi:10.1016/j.trac.2010.11.016
  • [19]. Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M., Rice-Evans, C. (1999). Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic Biol Med., 26 (9–10), 1231-1237.
  • [20]. Aliyazicioglu, R., Eyupoglu, O.E., Sahin, H., Yildiz, O., Baltas, N. (2013). Phenolic components, antioxidant activity, and mineral analysis of Capparis spinosa L. Afr. J. Biotechnol., 12(47), 6643-6649, doi: 10.5897/AJB2013.13241
  • [21]. Nimmano, N., Somavarapu, S., Taylor, K.M.G. (2018). Aerosol characterisation of nebulised liposomes co-loaded with erlotinib and genistein using an abbreviated cascade impactor method. Int J Pharmaceut., 542(1-2), 8–17, doi:10.1016/j.ijpharm.2018.02.035
  • [22]. Rock, F., Barsan, N., Weimar, U. (2008). Electronic Nose: Current Status and Future Trends. Chem Rev., 108(2), 705–725, doi:10.1021/cr068121q
  • [23]. Lee, H.-H., Kim, I.-J., Kang, S.-T., Kim, Y.-H., Lee, J.-O., Ryu, C.-H. (2010). Development of black garlic yakju and its antioxidant activity. Korean Journal of Food Science and Technology, 42(1), 69-74.
  • [24]. Kim, J.S, Kang, O.J, Gweon, O.C. (2013). Comparison of phenolic acids and flavonoids in black garlic at different thermal processing steps. J Funct Foods, 51, 80-86.
  • [25]. Wang, W., Sun, Y. (2016). In vitro and in vivo antioxidant activities of polyphenol extracted from black garlic. Food Sci Technol., 37(4), 681-685, doi:10.1590/1678-457X.30816
  • [26]. Molina-Calle, M., Priego-Capote, F., Luque de Castro, M.D. (2017). Headspace GC-MS volatile profile of black garlic vs fresh garlic: Evolution along fermentation and behavior under heating, Food Sci Technol., 80, 98-105, doi:10.1016/j.lwt.2017.02.010
  • [27]. Gorinstein, S., Leontowicz, H., Leontowicz, M., Namiesnik, J., Najman, K., Drzewiecki J., et al. (2008). Comparison of the Main Bioactive Compounds and Antioxidant Activities in Garlic and White and Red Onions after Treatment Protocols. J. Agric. Food Chem., 56 (12), 4418-4426, doi:10.1021/jf800038h
  • [28]. Tamaki, K., Sonoki, S., Tamaki, T., & Ehara, K. (2008). Measurement of odour after in vitro or in vivo ingestion of raw or heated garlic, using electronic nose, gas chromatography and sensory analysis. Int J Food Sci Technol., 43, 130–139, doi:10.1111/j.1365 2621.2006.01403.x
  • [29]. Koca, I., Tekguler, B., & Koca, A. F. (2016). Some physical and chemical characteristics of Taşköprü and Chinese black garlics. Acta Horticulturae, 1143, 221–226, doi:10.17660/actahortic.2016.1143.32
  • [30]. Chen, Y.-A., Tsai, J.-C., Cheng, K.-C., Liu, K.-F., Chang, C.-K., & Hsieh, C.-W. (2018). Extracts of black garlic exhibits gastrointestinal motility effect. Food Res Int., 107, 102–109, doi:10.1016/j.foodres.2018.02.003
  • [31]. Shin, J.-H., Lee, H.-G., Kang, M.-J., Lee, S.-J., Sung, N.-J. (2010). Antioxidant activity of solvent fraction from black garlic. J Korean Soc Food Sci Nutr, 39 (7), 933-940, doi:10.3746/jkfn.2010.39.7.933
  • [32]. Xiong, F, Dai, C-H., Hou, F-R., Zhu P-P., He R-H., and Ma, H-L. (2018). Study on the Ageing Method and Antioxidant Activity of Black Garlic Residues. Czech J. Food Sci., 36(1), 88–97, doi:10.17221/420/2016-CJFS
  • [33]. Martínez-Casas, L., Lage-Yusty, M., & López-Hernández, J. (2017). Changes in the Aromatic Profile, Sugars, and Bioactive Compounds When Purple Garlic Is Transformed into Black Garlic. J Agric Food Chem., 65 (49), 10804–10811, doi:10.1021/acs.jafc.7b04423
Primary Language en
Subjects Biology
Published Date June
Journal Section Articles
Authors

Orcid: 0000-0002-4449-0537
Author: Ozan Emre Eyupoglu (Primary Author)
Institution: ISTANBUL MEDIPOL UNIVERSITY
Country: Turkey


Dates

Publication Date : July 15, 2019

Bibtex @research article { ijsm564813, journal = {International Journal of Secondary Metabolite}, issn = {}, eissn = {2148-6905}, address = {Prof. Dr. İzzet KARA}, publisher = {İzzet KARA}, year = {2019}, volume = {6}, pages = {154 - 161}, doi = {10.21448/ijsm.564813}, title = {Antioxidant Activities, Phenolic Contents and Electronic Nose Analysis of Black Garlic}, key = {cite}, author = {Eyupoglu, Ozan Emre} }
APA Eyupoglu, O . (2019). Antioxidant Activities, Phenolic Contents and Electronic Nose Analysis of Black Garlic. International Journal of Secondary Metabolite , 6 (2) , 154-161 . DOI: 10.21448/ijsm.564813
MLA Eyupoglu, O . "Antioxidant Activities, Phenolic Contents and Electronic Nose Analysis of Black Garlic". International Journal of Secondary Metabolite 6 (2019 ): 154-161 <http://ijsm.ijate.net/en/issue/44217/564813>
Chicago Eyupoglu, O . "Antioxidant Activities, Phenolic Contents and Electronic Nose Analysis of Black Garlic". International Journal of Secondary Metabolite 6 (2019 ): 154-161
RIS TY - JOUR T1 - Antioxidant Activities, Phenolic Contents and Electronic Nose Analysis of Black Garlic AU - Ozan Emre Eyupoglu Y1 - 2019 PY - 2019 N1 - doi: 10.21448/ijsm.564813 DO - 10.21448/ijsm.564813 T2 - International Journal of Secondary Metabolite JF - Journal JO - JOR SP - 154 EP - 161 VL - 6 IS - 2 SN - -2148-6905 M3 - doi: 10.21448/ijsm.564813 UR - https://doi.org/10.21448/ijsm.564813 Y2 - 2019 ER -
EndNote %0 International Journal of Secondary Metabolite Antioxidant Activities, Phenolic Contents and Electronic Nose Analysis of Black Garlic %A Ozan Emre Eyupoglu %T Antioxidant Activities, Phenolic Contents and Electronic Nose Analysis of Black Garlic %D 2019 %J International Journal of Secondary Metabolite %P -2148-6905 %V 6 %N 2 %R doi: 10.21448/ijsm.564813 %U 10.21448/ijsm.564813
ISNAD Eyupoglu, Ozan Emre . "Antioxidant Activities, Phenolic Contents and Electronic Nose Analysis of Black Garlic". International Journal of Secondary Metabolite 6 / 2 (July 2019): 154-161 . https://doi.org/10.21448/ijsm.564813
AMA Eyupoglu O . Antioxidant Activities, Phenolic Contents and Electronic Nose Analysis of Black Garlic. IJSM. 2019; 6(2): 154-161.
Vancouver Eyupoglu O . Antioxidant Activities, Phenolic Contents and Electronic Nose Analysis of Black Garlic. International Journal of Secondary Metabolite. 2019; 6(2): 161-154.