Year 2018, Volume 5, Issue 4, Pages 353 - 361 2018-12-29

DNA barcoding and phytochemical profiling of wild plant "Lal lat tan" from Imugan, Sta Fe, Nueva Vizcaya, Philippines

John Dave C. Aquino [1] , Lariza Mae Pajarillaga [2] , Jerwin R. Undan [3]

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The Philippines hosts a wide-ranging diversity of plant species with pharmacological potential. Species identification on the basis of DNA sequences has been done for some time in plants and used to detect variations among the sequences specifying genetic divergence as a result of molecular evolution during the course of time. In this study, molecular approach as well as phylogentic analysis were conducted to identify the wild plant "Lal lat tan" collected from Imugan, Sta Fe, Nueva Vizcaya. In addition, thin layer chromatography was conducted to determine the bioactive compounds present in the wild plant. For molecular approach, the genomic DNA was extracted from the young leaves using CTAB and amplified using the nrDNA ITS marker. The PCR amplified product was sequenced and subjected for search query analysis using BLAST and was identified as Dendrocnide meyeniana. Using chloroform+methanol as solvent system, the result revealed the presence of saponins, phenols, tannins, flavonoids, anthrones, anthraquinones, terpenes and steroids which are considered as active medicinal phytochemical constituents. Lal lat tan is a wild plant and was known for having stinger leaf that lead to severe itchiness and swelling of eyes and face when touched.
ITS, Lal lat tan, Dendrocnide meyeniana
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Primary Language en
Subjects Biology
Published Date December
Journal Section Articles
Authors

Author: John Dave C. Aquino
Institution: Central Luzon State University
Country: Philippines


Author: Lariza Mae Pajarillaga
Institution: Central Luzon State University
Country: Philippines


Orcid: orcid.org/0000-0002-3657-1732
Author: Jerwin R. Undan (Primary Author)
Institution: Central Luzon State University
Country: Philippines


Bibtex @research article { ijsm493809, journal = {International Journal of Secondary Metabolite}, issn = {}, eissn = {2148-6905}, address = {İzzet KARA}, year = {2018}, volume = {5}, pages = {353 - 361}, doi = {10.21448/ijsm.493809}, title = {DNA barcoding and phytochemical profiling of wild plant "Lal lat tan" from Imugan, Sta Fe, Nueva Vizcaya, Philippines}, key = {cite}, author = {Aquino, John Dave C. and Pajarillaga, Lariza Mae and Undan, Jerwin R.} }
APA Aquino, J , Pajarillaga, L , Undan, J . (2018). DNA barcoding and phytochemical profiling of wild plant "Lal lat tan" from Imugan, Sta Fe, Nueva Vizcaya, Philippines. International Journal of Secondary Metabolite, 5 (4), 353-361. DOI: 10.21448/ijsm.493809
MLA Aquino, J , Pajarillaga, L , Undan, J . "DNA barcoding and phytochemical profiling of wild plant "Lal lat tan" from Imugan, Sta Fe, Nueva Vizcaya, Philippines". International Journal of Secondary Metabolite 5 (2018): 353-361 <http://ijsm.ijate.net/issue/39967/493809>
Chicago Aquino, J , Pajarillaga, L , Undan, J . "DNA barcoding and phytochemical profiling of wild plant "Lal lat tan" from Imugan, Sta Fe, Nueva Vizcaya, Philippines". International Journal of Secondary Metabolite 5 (2018): 353-361
RIS TY - JOUR T1 - DNA barcoding and phytochemical profiling of wild plant "Lal lat tan" from Imugan, Sta Fe, Nueva Vizcaya, Philippines AU - John Dave C. Aquino , Lariza Mae Pajarillaga , Jerwin R. Undan Y1 - 2018 PY - 2018 N1 - doi: 10.21448/ijsm.493809 DO - 10.21448/ijsm.493809 T2 - International Journal of Secondary Metabolite JF - Journal JO - JOR SP - 353 EP - 361 VL - 5 IS - 4 SN - -2148-6905 M3 - doi: 10.21448/ijsm.493809 UR - https://doi.org/10.21448/ijsm.493809 Y2 - 2018 ER -
EndNote %0 International Journal of Secondary Metabolite DNA barcoding and phytochemical profiling of wild plant "Lal lat tan" from Imugan, Sta Fe, Nueva Vizcaya, Philippines %A John Dave C. Aquino , Lariza Mae Pajarillaga , Jerwin R. Undan %T DNA barcoding and phytochemical profiling of wild plant "Lal lat tan" from Imugan, Sta Fe, Nueva Vizcaya, Philippines %D 2018 %J International Journal of Secondary Metabolite %P -2148-6905 %V 5 %N 4 %R doi: 10.21448/ijsm.493809 %U 10.21448/ijsm.493809
ISNAD Aquino, John Dave C. , Pajarillaga, Lariza Mae , Undan, Jerwin R. . "DNA barcoding and phytochemical profiling of wild plant "Lal lat tan" from Imugan, Sta Fe, Nueva Vizcaya, Philippines". International Journal of Secondary Metabolite 5 / 4 (December 2018): 353-361. https://doi.org/10.21448/ijsm.493809