Year 2018, Volume 5, Issue 4, Pages 362 - 374 2018-12-29

Enhancement of stevioside production by using biotechnological approach in in vitro culture of Stevia rebaudiana

Meltem Bayraktar [1] , Elmira Naziri [2] , Fatih Karabey [3] , Ismail Hakki Akgun [4] , Erdal Bedir [5] , Bärbel Röck-Okuyucu [6] , Aynur Gürel [7]

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Stevia rebaudiana Bertoni, which is an important plant for the food and health sector, contains calorie-free natural sweet-tasting steviol glycosides (SGs). In the present study, the effects of different elicitors [methyl jasmonate (MeJA), salicylic acid (SA), or chitosan (CHI)] on the in vitro production of stevioside and rebaudioside A were carried out. For this purpose, 3-week-old in vitro plantlets were transferred into 250 mL flasks containing liquid woody plant medium (WPM) supplemented with MeJA, SA, or CHI at different concentrations (0, 50, 100, or 200 µM), and were exposed to these elicitors for 2 weeks. A HPLC method was developed to quantify the aforementioned SGs in the cultivated plantlets and all of the elicitor types and concentrations resulted in an increase in stevioside production ranged between 2.87 mg/g dry weight (DW) (Control) and 50.07 mg/g DW (100 µM MeJA). The highest number of shoot, node, leaf, leaf length, and biomass accumulation and shoot length were observed with application of 100 µM CHI and control, respectively. The present findings open new perspectives for increasing the stevioside production using a plant tissue culture system.
Chitosan, Elicitor, Methyl jasmonate, Salicylic acid, Steviol glycosides, Stevia rebaudiana
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Primary Language en
Subjects Biology
Published Date December
Journal Section Articles
Authors

Orcid: 0000-0002-7569-6925
Author: Meltem Bayraktar (Primary Author)
Institution: Kırşehir Ahi Evran University
Country: Turkey


Orcid: orcid.org/0000-0003-2483-2496
Author: Elmira Naziri
Institution: EGE UNIVERSITY

Orcid: orcid.org/0000-0003-0305-6192
Author: Fatih Karabey
Institution: EGE UNIVERSITY

Author: Ismail Hakki Akgun
Institution: EGE UNIVERSITY

Orcid: orcid.org/0000-0003-1262-063X
Author: Erdal Bedir
Institution: EGE UNIVERSITY

Author: Bärbel Röck-Okuyucu
Institution: CELAL BAYAR UNIVERSITY

Orcid: orcid.org/0000-0002-7002-9752
Author: Aynur Gürel
Institution: EGE UNIVERSITY

Bibtex @research article { ijsm496724, journal = {International Journal of Secondary Metabolite}, issn = {}, eissn = {2148-6905}, address = {İzzet KARA}, year = {2018}, volume = {5}, pages = {362 - 374}, doi = {10.21448/ijsm.496724}, title = {Enhancement of stevioside production by using biotechnological approach in in vitro culture of Stevia rebaudiana}, key = {cite}, author = {Bayraktar, Meltem and Naziri, Elmira and Karabey, Fatih and Akgun, Ismail Hakki and Bedir, Erdal and Röck-Okuyucu, Bärbel and Gürel, Aynur} }
APA Bayraktar, M , Naziri, E , Karabey, F , Akgun, I , Bedir, E , Röck-Okuyucu, B , Gürel, A . (2018). Enhancement of stevioside production by using biotechnological approach in in vitro culture of Stevia rebaudiana. International Journal of Secondary Metabolite, 5 (4), 362-374. DOI: 10.21448/ijsm.496724
MLA Bayraktar, M , Naziri, E , Karabey, F , Akgun, I , Bedir, E , Röck-Okuyucu, B , Gürel, A . "Enhancement of stevioside production by using biotechnological approach in in vitro culture of Stevia rebaudiana". International Journal of Secondary Metabolite 5 (2018): 362-374 <http://ijsm.ijate.net/issue/39967/496724>
Chicago Bayraktar, M , Naziri, E , Karabey, F , Akgun, I , Bedir, E , Röck-Okuyucu, B , Gürel, A . "Enhancement of stevioside production by using biotechnological approach in in vitro culture of Stevia rebaudiana". International Journal of Secondary Metabolite 5 (2018): 362-374
RIS TY - JOUR T1 - Enhancement of stevioside production by using biotechnological approach in in vitro culture of Stevia rebaudiana AU - Meltem Bayraktar , Elmira Naziri , Fatih Karabey , Ismail Hakki Akgun , Erdal Bedir , Bärbel Röck-Okuyucu , Aynur Gürel Y1 - 2018 PY - 2018 N1 - doi: 10.21448/ijsm.496724 DO - 10.21448/ijsm.496724 T2 - International Journal of Secondary Metabolite JF - Journal JO - JOR SP - 362 EP - 374 VL - 5 IS - 4 SN - -2148-6905 M3 - doi: 10.21448/ijsm.496724 UR - https://doi.org/10.21448/ijsm.496724 Y2 - 2018 ER -
EndNote %0 International Journal of Secondary Metabolite Enhancement of stevioside production by using biotechnological approach in in vitro culture of Stevia rebaudiana %A Meltem Bayraktar , Elmira Naziri , Fatih Karabey , Ismail Hakki Akgun , Erdal Bedir , Bärbel Röck-Okuyucu , Aynur Gürel %T Enhancement of stevioside production by using biotechnological approach in in vitro culture of Stevia rebaudiana %D 2018 %J International Journal of Secondary Metabolite %P -2148-6905 %V 5 %N 4 %R doi: 10.21448/ijsm.496724 %U 10.21448/ijsm.496724
ISNAD Bayraktar, Meltem , Naziri, Elmira , Karabey, Fatih , Akgun, Ismail Hakki , Bedir, Erdal , Röck-Okuyucu, Bärbel , Gürel, Aynur . "Enhancement of stevioside production by using biotechnological approach in in vitro culture of Stevia rebaudiana". International Journal of Secondary Metabolite 5 / 4 (December 2018): 362-374. https://doi.org/10.21448/ijsm.496724