Year 2019, Volume 6, Issue 2, Pages 162 - 171 2019-08-07

Adventitious roots formation for enhanced and sustainable production of antioxidants in Brassica oleracea var. acephala (Brassicaceae)

Muhammad Adil [1] , Bilal Haider Abbasi [2]

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Brassica oleracea var. acephala is listed as the healthiest vegetable due to its high valued secondary metabolites content and antioxidant potential. This study was conducted to establish adventitious roots (ARs) culture as an alternative and feasible production of antioxidant secondary metabolites. ARs were induced from cotyledon explants in commercially available Murashige and Skoog (MS) plant nutrient media, gelled with 0.8% phyto-agar and supplemented with different concentration (0.1 ̶ 1.5 mg·L-1) of auxins (α-Naphthalene acetic acid; NAA, or Indole acetic acid; IAA, or Indole-3-butyric acid; IBA). AR formation responses in MS media at varying concentrations (0 ̶ 50 g·L-1) of sucrose and initial media pH (4, 5.0, 5.8, 7 & 8) were also studied. The bioprocessing of ARs were studied in liquid MS media containing NAA (1.5 mg·L-1) as growth regulator. The growth curve, important antioxidants (phenols & flavonoids), and free radical scavenging potential of ARs were studied for a period of 9-weeks. The ARs at stationary phase (7-week) attained highest accumulation of phenols and flavonoids, which ultimately showed the highest reactive species scavenging potential. This study provides the base for production of B. oleraceae var. acephala secondary metabolites on large scale to strengthen the bio-based economy of developing world.
phenolics, Adventitious roots, flavonoids, antioxidants, auxin
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Primary Language en
Subjects Biology
Published Date June
Journal Section Articles
Authors

Orcid: 0000-0001-7328-1550
Author: Muhammad Adil (Primary Author)
Institution: International Center for Chemical and Biological sciences
Country: Pakistan


Author: Bilal Haider Abbasi
Institution: Quaid I Azam University Islamabad
Country: Iran


Bibtex @research article { ijsm530027, journal = {International Journal of Secondary Metabolite}, issn = {}, eissn = {2148-6905}, address = {İzzet KARA}, year = {2019}, volume = {6}, pages = {162 - 171}, doi = {10.21448/ijsm.530027}, title = {Adventitious roots formation for enhanced and sustainable production of antioxidants in Brassica oleracea var. acephala (Brassicaceae)}, key = {cite}, author = {Adil, Muhammad and Abbasi, Bilal Haider} }
APA Adil, M , Abbasi, B . (2019). Adventitious roots formation for enhanced and sustainable production of antioxidants in Brassica oleracea var. acephala (Brassicaceae). International Journal of Secondary Metabolite, 6 (2), 162-171. DOI: 10.21448/ijsm.530027
MLA Adil, M , Abbasi, B . "Adventitious roots formation for enhanced and sustainable production of antioxidants in Brassica oleracea var. acephala (Brassicaceae)". International Journal of Secondary Metabolite 6 (2019): 162-171 <http://ijsm.ijate.net/issue/44217/530027>
Chicago Adil, M , Abbasi, B . "Adventitious roots formation for enhanced and sustainable production of antioxidants in Brassica oleracea var. acephala (Brassicaceae)". International Journal of Secondary Metabolite 6 (2019): 162-171
RIS TY - JOUR T1 - Adventitious roots formation for enhanced and sustainable production of antioxidants in Brassica oleracea var. acephala (Brassicaceae) AU - Muhammad Adil , Bilal Haider Abbasi Y1 - 2019 PY - 2019 N1 - doi: 10.21448/ijsm.530027 DO - 10.21448/ijsm.530027 T2 - International Journal of Secondary Metabolite JF - Journal JO - JOR SP - 162 EP - 171 VL - 6 IS - 2 SN - -2148-6905 M3 - doi: 10.21448/ijsm.530027 UR - https://doi.org/10.21448/ijsm.530027 Y2 - 2019 ER -
EndNote %0 International Journal of Secondary Metabolite Adventitious roots formation for enhanced and sustainable production of antioxidants in Brassica oleracea var. acephala (Brassicaceae) %A Muhammad Adil , Bilal Haider Abbasi %T Adventitious roots formation for enhanced and sustainable production of antioxidants in Brassica oleracea var. acephala (Brassicaceae) %D 2019 %J International Journal of Secondary Metabolite %P -2148-6905 %V 6 %N 2 %R doi: 10.21448/ijsm.530027 %U 10.21448/ijsm.530027
ISNAD Adil, Muhammad , Abbasi, Bilal Haider . "Adventitious roots formation for enhanced and sustainable production of antioxidants in Brassica oleracea var. acephala (Brassicaceae)". International Journal of Secondary Metabolite 6 / 2 (August 2019): 162-171. https://doi.org/10.21448/ijsm.530027