Year 2019, Volume 6, Issue 2, Pages 137 - 153 2019-08-07

Effect of Nitrogen, Phosphorus and Medium pH to Enhance Alkaloid Production from Catharanthus roseus Cell Suspension Culture

Malay Ranjan Mishra Mishra [1] , Rajesh Kumar Srivastava [2] , Nasim Akhtar [3]

10 103

Several elevated levels of nitrogen and phosphate at varying pH of the medium which impart a major influence on callus and biomass development and subsequent production of alkaloids was investigated using suspension culture system of Catharanthus roseus in the present study. The B5 medium was buffered at pH 4.51, 5.82 and 7.32 by addition of different levels of (A) diammonium hydrogen phosphate (NH4)2HPO4 and (B) ammonium dihydrogen orthophosphate (NH4H2PO4) representing the enhanced and varied supply of total nitrogen (NH4++NO3ˉ) and phosphate compared to MS medium (as control) for cell biomass production and alkaloid yield. The pH of the medium have shown significant effects with maximum biomass fresh wt., dry wt. and total alkaloid yield at 5.82 medium pH with elevated phosphate levels and total nitrogen concentration of 3710.10 mg/L compared to control MS medium with 2850 mg/L total nitrogen. At 3667.33 and 3752.48 mg/L of total nitrogen with enhanced phosphate supply showed reduced biomass fresh wt., dry wt. and total alkaloid yield at lower (4.51) and higher (7.32) medium pH respectively. Inclusion of 200 mg/L of tryptophan or phenylalanine as reduced nitrogen source in B5 medium buffered at 5.82 ± 0.2 pH showed enhanced biomass and alkaloid production. Hence, addition of nitrogen, phosphate, tryptophan, phenylalanine as nutrient in suspension culture stimulate their uptake to enhance cell biomass and total alkaloids production but as a function of pH of the medium.
Alkaloid, Catharanthus roseus, medium pH, Nitrogen, Phenylalanine, Phosphorus, Tryptophan
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Primary Language en
Subjects Biology
Journal Section Articles

Orcid: 0000-0003-1714-0061
Author: Malay Ranjan Mishra Mishra
Institution: GITAM Institute of Technology
Country: India

Author: Rajesh Kumar Srivastava
Institution: GITAM Institute of Technology
Country: India

Author: Nasim Akhtar (Primary Author)
Institution: GITAM Institute of Technology
Country: India

Bibtex @research article { ijsm559679, journal = {International Journal of Secondary Metabolite}, issn = {}, eissn = {2148-6905}, address = {İzzet KARA}, year = {2019}, volume = {6}, pages = {137 - 153}, doi = {10.21448/ijsm.559679}, title = {Effect of Nitrogen, Phosphorus and Medium pH to Enhance Alkaloid Production from Catharanthus roseus Cell Suspension Culture}, key = {cite}, author = {Mishra, Malay Ranjan Mishra and Srivastava, Rajesh Kumar and Akhtar, Nasim} }
APA Mishra, M , Srivastava, R , Akhtar, N . (2019). Effect of Nitrogen, Phosphorus and Medium pH to Enhance Alkaloid Production from Catharanthus roseus Cell Suspension Culture. International Journal of Secondary Metabolite, 6 (2), 137-153. DOI: 10.21448/ijsm.559679
MLA Mishra, M , Srivastava, R , Akhtar, N . "Effect of Nitrogen, Phosphorus and Medium pH to Enhance Alkaloid Production from Catharanthus roseus Cell Suspension Culture". International Journal of Secondary Metabolite 6 (2019): 137-153 <>
Chicago Mishra, M , Srivastava, R , Akhtar, N . "Effect of Nitrogen, Phosphorus and Medium pH to Enhance Alkaloid Production from Catharanthus roseus Cell Suspension Culture". International Journal of Secondary Metabolite 6 (2019): 137-153
RIS TY - JOUR T1 - Effect of Nitrogen, Phosphorus and Medium pH to Enhance Alkaloid Production from Catharanthus roseus Cell Suspension Culture AU - Malay Ranjan Mishra Mishra , Rajesh Kumar Srivastava , Nasim Akhtar Y1 - 2019 PY - 2019 N1 - doi: 10.21448/ijsm.559679 DO - 10.21448/ijsm.559679 T2 - International Journal of Secondary Metabolite JF - Journal JO - JOR SP - 137 EP - 153 VL - 6 IS - 2 SN - -2148-6905 M3 - doi: 10.21448/ijsm.559679 UR - Y2 - 2019 ER -
EndNote %0 International Journal of Secondary Metabolite Effect of Nitrogen, Phosphorus and Medium pH to Enhance Alkaloid Production from Catharanthus roseus Cell Suspension Culture %A Malay Ranjan Mishra Mishra , Rajesh Kumar Srivastava , Nasim Akhtar %T Effect of Nitrogen, Phosphorus and Medium pH to Enhance Alkaloid Production from Catharanthus roseus Cell Suspension Culture %D 2019 %J International Journal of Secondary Metabolite %P -2148-6905 %V 6 %N 2 %R doi: 10.21448/ijsm.559679 %U 10.21448/ijsm.559679
ISNAD Mishra, Malay Ranjan Mishra , Srivastava, Rajesh Kumar , Akhtar, Nasim . "Effect of Nitrogen, Phosphorus and Medium pH to Enhance Alkaloid Production from Catharanthus roseus Cell Suspension Culture". International Journal of Secondary Metabolite 6 / 2 (August 2019): 137-153.